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Influence of Lauric Acid Supplementation on Transportation-Induced Stress in Zebrafish (Danio rerio)
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Amir Mohammad Khajavi *   , Roghie Safari , Hossein Hosseinifar , Matin Yashizi , Hamed Azadi |
| Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran |
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Abstract: (96 Views) |
The present study investigates the effects of supplementing different levels of lauric acid in the diet of zebrafish (Danio rerio) on biochemical indices and internal responses following exposure to transport stress. A total of 324 zebrafish with an average weight of 0.22±0.01 g was divided into four experimental groups: Group 1 (control, no lauric acid added to the diet), Group 2 (0.5 g lauric acid per kg of feed), Group 3 (1 g lauric acid per kg of feed), and Group 4 (2 g lauric acid per kg of feed). The fish were fed these diets for eight weeks. At the end of the experiment, the fish were subjected to three hours of transport stress, after which biochemical indices, liver enzymes, and stress responses were evaluated. Results indicated a significant reduction in blood glucose and cholesterol levels in the lauric acid-treated groups compared to the control (P<0.05). No significant difference in triglyceride levels was observed between the LA0.5 and LA1 treatments and the control group (P>0.05); however, the LA2 treatment showed a significant decrease relative to the control (P<0.05). Examination of liver enzyme activity revealed that although the LA0.5 treatment did not differ significantly from the control (P > 0.05), it exhibited lower liver enzyme levels compared to other lauric acid treatments, while the LA2 treatment had higher enzyme levels than the other groups. Cortisol hormone levels showed that the LA0.5 treatment significantly reduced cortisol compared to the control (P<0.05), whereas no significant differences were found in the LA1 and LA2 treatments (P>0.05). Based on these findings, incorporating 0.5 g/kg lauric acid in the diet may serve as a nutritional strategy to reduce stress markers, and modulate biochemical metabolic parameters, thereby optimizing physiological performance and enhancing the quality of zebrafish culture.
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| Keywords: Lauric acid, Stress, Biochemical indices, Transport, Zebrafish. |
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Full-Text [PDF 955 kb]
(33 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2025/07/19 | Accepted: 2025/07/1 | Published: 2025/07/19
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