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:: Volume 12, Issue 3 (10-2025) ::
3 2025, 12(3): 23-33 Back to browse issues page
Effects of Salep (Orchis mascula) on biochemical indices and transportation stress responses of zebrafish (Danio rerio)
Abstract:   (34 Views)

This study investigated the effects of incorporating Salep, Orchis mascula into the diet of zebrafish (Danio rerio) on physiological indices and stress responses following transport stress. A total of 288 zebrafish with an average weight of 0.23 ± 0.01 g, were fed four experimental diets for 8 weeks: a control diet (C, basal diet without Salep) and three Salep-supplemented diets, including OM0.5 (0.5%), OM1 (1%), and OM2 (2%). At the end of the experiment, fish were subjected to transport stress two one hour, and biochemical indices, hepatic enzyme activities, and stress responses were evaluated. Results showed that diets containing 1% and 2% Salep significantly reduced plasma glucose, cortisol, cholesterol, lactate dehydrogenase, and aspartate aminotransferase activity (P < 0.05), while alkaline phosphatase activity peaked in the OM1 group (P < 0.05). However, triglyceride levels and alanine aminotransferase activity increased in the 2% group (P < 0.05). Based on these findings, using 1% Salep supplementation is recommended to enhance hepatic health, reduce stress, and regulate biochemical metabolism in zebrafish aquaculture.




 
Keywords: Hepatic enzymes, Stress response, Salep, Medicinal herbs, Zebrafish
Full-Text [PDF 798 kb]   (14 Downloads)    
Type of Study: Research | Subject: Special
Received: 2025/04/30 | Accepted: 2025/10/2 | Published: 2025/09/7
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Effects of Salep (Orchis mascula) on biochemical indices and transportation stress responses of zebrafish (Danio rerio). 3 2025; 12 (3) :23-33
URL: http://ornamentalaquatics.ir/article-1-382-en.html


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Volume 12, Issue 3 (10-2025) Back to browse issues page
مجله علمی آبزیان زینتی Journal of Ornamental Aquatics
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