Successful reproduction and rearing of marine ornamental fish significantly depend on capturing, digesting, and assimilating sufficient amounts of live food. The most limiting factor in producing marine ornamental fish is the requirement for appropriately sized live food as a first feed. This issue is due to the small mouth size of many high commercial-value species. Therefore, the need for prey with the right size is one of the necessities in producing marine ornamental fish. In this regard, development and progress in the usage of new live food are required to expand this industry. The most common live food used in the rearing of marine ornamental fish larvae is rotifer and Artemia. These prey are not ideal in terms of size and especially nutritional aspects. Significant improvements in rearing marine ornamental fish larvae can be achieved by providing copepods and ciliates to feed them. Currently, various species of copepods can be successfully cultivated. The participation of copepods in the nutritional scheme of larvae, as exclusive feed or as a supplement to traditional prey such as rotifer and Artemia, is beneficial due to their high nutritional quality and a wider range of available sizes for different stages of larval development. Due to their small size, ciliates are a suitable food source for feeding the larvae of ornamental marine fish with very small mouth sizes. They have the characteristics of an effective live food, such as rapid reproduction, tolerance to high-density cultures, consumption of various food items, and the possibility of enrichment.