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:: Volume 5, Issue 1 (4-2018) ::
3 2018, 5(1): 21-27 Back to browse issues page
Ovary culture of Goldfish Carassius auratus
Mohammad Reza Nowruzfashkhami , Mahmoud Bahmani
assistant professor Agricultural Research, Education and Extension Organization
Abstract:   (240 Views)
In this study primary culture, subculture1 and subculture2 of ovarian tissue from goldfish was done to develop an in vitro system of ovarian cells in this species. A goldfish weighing 42g and 7 cm in length was anesthetized by Clove powder (0.2 g/lit) and its ovary was removed and put in a petri dish containing 5ml L-15 medium, Streptomycin sulphate (200 ug/ml), Penicillin G potassium (Gibco, 200 iu/ml) and Amphotericin B (5 ug/ml). The oocytes which were in advanced maturation stage (IV) were isolated from ovary and the ovary cut into small pieces (explants) by a 5ml syringe. The ovary explants cultivated in 5ml L-15 medium supplemented with 15% FBS, Streptomycin sulphate (100 ug/ml), Penicillin G potassium (Gibco, 100 iu/ml) and Amphotericin B (2.5 ug/ml) at  25°C. After two weeks when confluent monolayer formed on the bottom of the flask, the cells were washed with PBS containing antibiotics, dislodged by Trypsin-EDTA solution (0.25%) and cultured in 5ml of L-15 medium, FBS (15%), antibiotics at 25 °C (first subculture). After confluent monolayer formation, on the sixteenth day similar procedure was followed for the second subculture.The emerging cells from explants and two subcultures exhibited fibroblast-like cells.
Keywords: Ovary culture, Goldfish, Carassius auratus
Full-Text [PDF 627 kb]   (138 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/12/8 | Accepted: 2018/04/24 | Published: 2018/04/24
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Nowruzfashkhami M R, Bahmani M. Ovary culture of Goldfish Carassius auratus. 3. 2018; 5 (1) :21-27
URL: http://ornamentalaquatics.ir/article-1-161-en.html


Volume 5, Issue 1 (4-2018) Back to browse issues page
مجله علمی-ترویجی آبزیان زینتی Journal of Ornamental Aquatics
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