It is now more than 10 years that scientists start DNA barcoding for species and still fish DNA barcoding is in infancy stage. International Barcode of Life states that ‘‘DNA sequence can be used to identify different species, in the same way, a supermarket scanner can use a familiar black strip of the UPC barcode to identify purchases’’. So, we can identify the fish species quickly with the help of the barcode reader. Reliable recovery of the 5′region of the cytochrome c oxidase I (COI) gene is critical for the ongoing effort to gather DNA barcodes for all fish species. It may help to develop fish DNA barcoding more easily as well as quickly. In other respect Species hybridization and very recently radiated species can represent a problem for barcoding. Supplementary studies show that problems with the accuracy of taxonomic identifications are more important than these tow subjects and DNA barcoding can help to correct misidentification. Finally, barcoding would be useful for regulating marine ornamental fish trade/export/imports there by contributing to the effective management of resources.