In order to improve fish health and reduce the use of chemical drugs due to the unwanted side effects of these substances in the prevention and treatment of aquatic diseases, the effect of various food supplementations on immune system has been investigated. It is necessary to consider the suitable nutrition and immunostimulant feed additive to maintain the natural growth and health of aquatic species. Over the past two decades, researchers focused on immunostimulants to reduce stress and diseases, as well as increasing the safety and overall fish health. The use of immunostimulants is a unique method in aquaculture to control and prevent disease and improve the cellular and humeral immune system. These dietary supplements can directly improve immune function by effecting the genes responsible for immune and inflammatory. This study refers to some common immunostimulants in aquaculture and their effects on the expression of immune related genes.