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The study of the population structure of the white fin fish Chirocentrus nudus (Swinson, 1839) in the coasts of the Persian Gulf with morphometry and DNA barcoding methods
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Hakime Fekrandish *   |
| islamic azad university of bushehr |
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Abstract: (333 Views) |
| In order to study the genetic structure and morphology of the population of the whitefin wolf herring (Chirocentrus nudus) from three regions of Abadan, Shif and Bandar Abbas on the coast of the Persian Gulf, 20 specimens from each station and a total of 60 fish were collected using gill-nets in May 2019. 29 morphometric and 5 counting traits were measured. For genetic study using mitochondrial cytochrome oxidase (COI) gene sequencing method, a piece of caudal fin of 6 fish samples from Shif, Abadan and Bandar Abbas stations were fixed in 96% ethanol and transferred to the laboratory. DNA extraction was done by phenol chloroform and polymerase chain reaction with a pair of primers. After sequencing or COI gene barcode, the sequences were aligned. The results of sequencing showed that the amplified fragment is exactly 595 bp. The COI gene in the region studied is exactly the same in all samples. Also, the results of principal component analysis (PCA) for morphometric traits showed that 9 factors with eigenvalues greater than 1 were selected, which includes 84.56% of the variation of morphometric traits. The distribution diagram based on the first and second components, dendrogram of morphometric traits and phylogeny tree drawing with Maximum likelihood, Neighbor-joining and UPGMA software showed that the whitefin wolf herring has at least two populations in the north of the Persian Gulf. |
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| Keywords: Whitefin wolf herring, Morphometry, Sequencing, COI gene barcode |
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Full-Text [PDF 928 kb]
(77 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2024/06/2 | Accepted: 2024/04/29 | Published: 2024/08/3
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