fa مقاله علمی – پژوهشی:‌ مطالعه ساختار جمعیت ماهی خارو باله سفید 1839) Chirocentrus nudus (Swinson, در سواحل خلیج فارس با روش‌های ریخت‌سنجی و DNA Barcoding تخصصي Special پژوهشي Research <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">برای مطالعه ساختار ژنتیکی و ریخت سنجی جمعیت <span style="background:white"><span style="color:black">ماهی خارو باله سفید</span></span></span></span> <i><span dir="LTR" style="background:white"><span style="color:black">Chirocentrus nudus</span></span></i> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">&nbsp;از سه منطقه آبادان، شیف و بندرعباس در سواحل خلیج فارس، از هر ایستگاه 20 عدد و در</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">مجموع</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">60 قطعه</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">ماهی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">خارو باله سفید با تور گوشگیر در اردیبهشت ماه 1399 صید گردید. 29</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">صفت</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">ریخت</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">‌‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">سنجی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">و</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">5</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">صفت شمارشی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">اندازه</span></span>‌<span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">گیری</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">گردید.<span style="background:white"><span style="color:black"> برای مطالعه ژنتیکی </span></span>به روش </span></span><a href="#_ftn1" name="_ftnref1" title=""><span class="MsoFootnoteReference" style="vertical-align:super"><span class="MsoFootnoteReference" style="vertical-align:super"><span style="font-size:11.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">[1]</span></span></span></span></span></a>DNA Barcoding<span style="font-size:12.0pt"><span b="" compset="" style="font-family:"> از طریق توالی‌‌یابی ژن سیتوکروم اکسیداز میتوکندری&nbsp; (</span></span>COI<span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">)<span style="background:white"><span style="color:black"> نیز قطعه‌ای از باله دمی 6 نمونه ماهی از هر ایستگاه شیف، آبادان و بندرعباس، در اتانول 96 درصد قرار داده شد </span></span>و به آزمایشگاه منتقل گردید. ا<span style="background:white"><span style="color:black">ستخراج</span></span></span></span><span dir="LTR" style="background:white"><span style="color:black"> DNA </span></span><span lang="FA" style="font-size:12.0pt"><span style="background:white"><span b="" compset="" style="font-family:"><span style="color:black">به روش فنل کلروفروم &nbsp;و با واکنش زنجیره‌ای پلی‌مراز با یک جفت پرایمر انجام گرفت. بعد از توالی‌یابی یا بارکد ژن</span></span></span></span><span dir="LTR" style="background:white"><span style="color:black"> COI </span></span><span lang="FA" style="font-size:12.0pt"><span style="background:white"><span b="" compset="" style="font-family:"><span style="color:black">، ردیف کردن توالی ها صورت گرفت. </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">نتایج حاصل از تعیین توالی نشان داد که قطعه تکثیر شده 595 جفت باز طول دارد. ژن </span></span>COI <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">&nbsp;در ناحیه‌ای که مورد مطالعه قرار گرفته بوده، در تمام نمونه‌ها- مشابه همدیگر بود. &nbsp;همچنین نتایج</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">آنالیز</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">تجزیه</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">به</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">مولفه</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">‌‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">های اصلی</span></span>(PCA) <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">&nbsp;برای صفات ریخت</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">‌‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">سنجی نشان</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">داد که تعداد 9 عامل با مقادیر ویژه بزرگ</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">‌‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">تر از 1 انتخاب شدند که 56/84 درصد شامل تنوع صفات ریخت</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">‌‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">سنجی است. نمودار پراکنش بر اساس مؤلفه</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">‌‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">های اول و دوم، دندروگرام صفات ریخت‌سنجی و ترسیم درخت فیلوژنی با نرم افزار </span></span>Maximum linkhood<span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:">، </span></span>Neighbor- joining<span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> و </span></span>UPGMA<span lang="FA" style="font-size:12.0pt"><span b="" compset="" style="font-family:"> نشان داد <span style="background:white"><span style="color:black">که ماهی خاروباله سفید در شمال خلیج فارس دارای حداقل دو جمعیت است. </span></span></span></span></span></span></span></span> <div>&nbsp; <hr align="left" size="1" width="33%" > <div id="ftn1"><span style="font-size:10pt"><span style="font-family:Calibri,sans-serif"><a href="#_ftnref1" name="_ftn1" title=""><span class="MsoFootnoteReference" style="vertical-align:super"><span new="" roman="" style="font-family:" times=""><span class="MsoFootnoteReference" style="vertical-align:super"><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">[1]</span></span></span></span></span></span></a><span new="" roman="" style="font-family:" times=""> Biological identifications through DNA barcodes (DNA Barcoding)</span><span style="font-family:"Times New Roman",serif"></span></span></span></div> </div> <span style="font-size:11pt"><span style="line-height:17.0pt"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">In order to study the genetic structure and morphology of the population of </span></span><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">the </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">whitefin wolf herring</span></span><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> (<i>Chirocentrus nudus</i>)</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> from three regions of Abadan, Shif and Bandar Abbas on the coast of the Persian Gulf, 20 specimens from each station and a total of 60 fish were collected using gill-nets in May 2019. 29 morphometric and 5 counting traits were measured. For genetic study using mitochondrial cytochrome oxidase (COI) gene sequencing method, a piece of caudal fin of 6 fish samples from Shif, Abadan and Bandar Abbas stations were fixed in 96% ethanol and transferred to the laboratory. DNA extraction was done by phenol chloroform and polymerase chain reaction with a pair of primers. After sequencing or COI gene barcode, the sequences were aligned. The results of sequencing showed that the amplified fragment is exactly 595 bp. The COI gene in the region studied is exactly the same in all samples. Also, the results of principal component analysis (PCA) for morphometric traits showed that 9 factors with eigenvalues ​​greater than 1 were selected, which includes 84.56% of the variation of morphometric traits. The distribution diagram based on the first and second components, dendrogram of morphometric traits and phylogeny tree drawing with Maximum likelihood, Neighbor-joining and UPGMA software showed that the whitefin wolf herring</span></span> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">has at least two populations in the north of the Persian Gulf.</span></span></span></span></span></span> خارو باله سفید, ریخت‌‌سنجی, توالی‌‌یابی, بارکد ژن COI Whitefin wolf herring, Morphometry, Sequencing, COI gene barcode 17 29 http://ornamentalaquatics.ir/browse.php?a_code=A-10-282-1&slc_lang=fa&sid=1 Hakime Fekrandish حکیمه فکراندیش hfekrandish@yahoo.com 10031947532846002873 10031947532846002873 Yes islamic azad university of bushehr دانشگاه آزاد اسلامی واحد بوشهر