The aim of hybridization is to get the generation which has better characters than the parent. Because of pretty apparent and different species, ornamental fish attract peoples, attention over the world and there was the limited study about them so the purpose of this study was to investigate the hybridization between guppy and molly, growth performance and reproduction indices next generation. Virgin females and males aged 2 weeks were used in the experiment. 4 females and 2 males were stored per a glass aquarium (40×30×30). Fish were randomly stored in 4 treatments and 3 repeats including male guppy and female molly, male molly and female guppy, male guppy and female guppy and male molly and female molly and fed biomar. At the end of the trial, the possibility of molly and guppy hybridization was evaluated. The results indicated that no pregnancy and larva were seen in prospective treatments.